Labeling of DNA via rearrangement of S-2-aminoethyl phosphorothioates to N-(2-mercaptoethyl)phosphoramidates.
نویسندگان
چکیده
The reaction of phosphorothioates in DNA with 2-bromoethylammonium bromide results in S-2-aminoethyl phosphorothioates, which can rearrange to N-(2-mercaptoethyl)phosphoramidates providing a facile method for the generation of site-specific thiol labeling of DNA sequences. The applicability of this method was demonstrated by conjugation of the thiolated DNA sequence with Na-(3-maleimidylpropionyl) biocytin and Alexa Fluor 546 C5-maleimide.
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ورودعنوان ژورنال:
- Organic & biomolecular chemistry
دوره 6 5 شماره
صفحات -
تاریخ انتشار 2008